Figure 9
From: Toll-like receptor 2 agonists inhibit human fibrocyte differentiation
Conditioned media from PBMCs incubated with LM-MS, PG-LPS and LTA inhibit fibrocyte differentiation. (A). Human PBMCs were cultured for 5 days in the presence of the TLR2 agonists Pam3CSK4, HKLM, LM-MS, PG-LPS, LTA and FSL-1, the TLR4 agonist LPS, and the NLR agonist PGN, using the same concentrations as in Figure 6. SFM was used as a buffer control, and LPS and PGN were used as negative controls, since these agonists do not inhibit fibrocyte differentiation. Conditioned media were removed on day 3 from duplicates of these wells. The results are means ± SEM of fibrocytes per 2.5 × 105 PBMCs (n = 5 separate experiments). *P < 0.05, **P < 0.01, and ***P < 0.001 compared to control (one-way ANOVA, Dunnett's test). (B) Human monocytes were cultured for 5 days in the presence of the conditioned media from day 3 PBMCs incubated with TLR2 agonists. The results are means ± SEM of fibrocytes per 2.5 × 105 PBMCs (n = 5 separate experiments). Conditioned media from PBMCs incubated with the TLR2 agonists LM-MS, PG-LPS and LTA significantly inhibited the differentiation of monocytes to fibrocytes. *P < 0.05 compared to control (one-way ANOVA, Dunnett's test).