Toll-like receptor 3 (TLR3) and TLR7 agonists do not affect fibrocyte differentiation. Human peripheral blood mononuclear cells (PBMCs) were cultured in different concentrations of the TLR3 agonist polyinosine-polycytidylic acid (Poly I:C) and the TLR7 agonists imiquimod (IMIQ), gardiquimod (GIMIQ) and loxoribine. After 5 days, the cells were air-dried, fixed and stained, and the number of fibrocytes was counted. The results are means ± SEM of fibrocytes per 2.5 × 105 PBMCs (n = 3 separate experiments). Although high concentrations of IMIQ and GIMIQ appear to decrease the number of fibrocytes compared to no addition, the decrease is not significant by either analysis of variance (ANOVA) or t-test. Arrows indicate the reported concentrations for the TLR agonists previously shown to be functional (Table 1).