Table 1 Primer sequences used for quantitative real-time RT-PCR.
| Gene | Primer pair Sequence (5'-3') |
Annealing temperature |
|---|---|---|
| TLR-2 | For: GGGTTGAAGCACTGGACAAT | 55°C |
| Rev: TTCTTCCTTGGAGAGGCTGA | ||
| TLR-3 | For: ACACCATCTATTAAAAGACCCATTAT | 62°C |
| Rev: TCCAGATTTTGTTCAATAGCTTGTT | ||
| TLR-4 | For: GGTCACCTTTTCTTGATTCCA | 55°C |
| Rev: TCAGAGGTCCATCAAACATCAC | ||
| TLR-7 | For: GCTATCAGATTCAAAAACAACAGAA | 55°C |
| Rev: CACAAACACCTTTGTAGATCACTTCT | ||
| TLR-8 | For: GGCTTTCTTTCTGAAGTCAGTAGTCT | 58°C |
| Rev: TTTCCGTGTAGTTCCAACATAGATAA | ||
| TLR-9 | For: CTGAGTGAGAACTTCCTCTACAAATG | 58°C |
| Rev: TCTTTTGGTAATTGAAGGACAGGTTA | ||
| β-Actin | For: CGGCATCGTCACCAACTG | 60°C |
| Rev: GGCACACGCAGCTCATTG |