Gene | Primer pair Sequence (5'-3') |
Annealing temperature |
---|---|---|
TLR-2 | For: GGGTTGAAGCACTGGACAAT | 55°C |
Rev: TTCTTCCTTGGAGAGGCTGA | ||
TLR-3 | For: ACACCATCTATTAAAAGACCCATTAT | 62°C |
Rev: TCCAGATTTTGTTCAATAGCTTGTT | ||
TLR-4 | For: GGTCACCTTTTCTTGATTCCA | 55°C |
Rev: TCAGAGGTCCATCAAACATCAC | ||
TLR-7 | For: GCTATCAGATTCAAAAACAACAGAA | 55°C |
Rev: CACAAACACCTTTGTAGATCACTTCT | ||
TLR-8 | For: GGCTTTCTTTCTGAAGTCAGTAGTCT | 58°C |
Rev: TTTCCGTGTAGTTCCAACATAGATAA | ||
TLR-9 | For: CTGAGTGAGAACTTCCTCTACAAATG | 58°C |
Rev: TCTTTTGGTAATTGAAGGACAGGTTA | ||
β-Actin | For: CGGCATCGTCACCAACTG | 60°C |
Rev: GGCACACGCAGCTCATTG |