Gene | Primer pair Sequence (5'-3') | Annealing temperature |
---|---|---|
TLR-2 | For: GGGTTGAAGCACTGGACAAT | 55°C |
 | Rev: TTCTTCCTTGGAGAGGCTGA |  |
TLR-3 | For: ACACCATCTATTAAAAGACCCATTAT | 62°C |
 | Rev: TCCAGATTTTGTTCAATAGCTTGTT |  |
TLR-4 | For: GGTCACCTTTTCTTGATTCCA | 55°C |
 | Rev: TCAGAGGTCCATCAAACATCAC |  |
TLR-7 | For: GCTATCAGATTCAAAAACAACAGAA | 55°C |
 | Rev: CACAAACACCTTTGTAGATCACTTCT |  |
TLR-8 | For: GGCTTTCTTTCTGAAGTCAGTAGTCT | 58°C |
 | Rev: TTTCCGTGTAGTTCCAACATAGATAA |  |
TLR-9 | For: CTGAGTGAGAACTTCCTCTACAAATG | 58°C |
 | Rev: TCTTTTGGTAATTGAAGGACAGGTTA |  |
β-Actin | For: CGGCATCGTCACCAACTG | 60°C |
 | Rev: GGCACACGCAGCTCATTG |  |