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Table 1 Sequences used for real-time quantitative polymerase chain reaction

From: Epithelial-mesenchymal transition in primary human bronchial epithelial cells is Smad-dependent and enhanced by fibronectin and TNF-α

Gene

Forward primer (5'-3')

Reverse primer (5'-3')

Probe

Collagen I

TGGCCTCGGAGGAAACTTT

TCCGGTTGATTTCTCATCATAGC

CCCCAGCTGTCTTAT

MMP-2

CGTCTGTCCCAGGATGACATC

TGTCAGGAGAGGCCCCATAG

6FAM-AGGGCATTCAGGAGC

MMP-9

TGGGCAGATTCCAAACCTTT

TCTTCCGAGTAGTTTTGGATCCA

6-FAM-CCACCACAACATCACCTA

E-cadherin

Pre-mixed primers and probe (Applied Biosystems, CA, USA)

  

GAPDH

Pre-mixed primers and probe (Applied Biosystems)

  
  1. MMP, matrix metalloprotease; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.
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Fibrogenesis & Tissue Repair

ISSN: 1755-1536

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