Figure 4

Absence of CCR7 is associated with pulmonary localization of CD4+, CD4+CD25+FoxP3+ and CD8+ T cells. (A, B) Lung cells and (C, D) bronchial lymph node cells from wild type (WT) and C-C chemokine receptor (CCR)7^-/- mice 1,3,7 and 21 days after bleomycin challenge were stained with (A, C) phycoerythrin (PE)-cyanin (Cy)7-conjugated anti-CD4 or (B, D)PE-conjugated anti-CD25 and PE-Cy5-conjugated anti-forkhead box (FOX)P3. CD4+CD25+FOXP3 T cells are expressed as percentage of total cells. n = three to mice/group/experiment. Samples were individualized except in those from the bronchial lymph nodes (brLNs) on days 3 and 21, in which cells from three to five mice were pooled. These data are representative of two separate experiments. TaqMan PCR analysis of whole lung samples for (E) interleukin (IL)-2 and (F) indoleamine 2,3 dioxygenase (IDO) transcript expression 21 days after bleomycin challenge. Transcript expression was compared with that before the intratracheal (IT) bleomycin challenge (that is, day 0). All data are shown as the expression fold difference from three to five mice at each time point after i.t. bleomycin challenge. SEM from four to five mice at each time point. ***P < 0.001. ND = not detected; NS = not significant.