Juglone reduced EMT and oxidative stress after unilateral ureteral obstruction (UUO). Right (control) and left (UUO) kidneys were removed after 2 weeks, fixed and stained with antibodies for E-cadherin (pink, epithelial marker) and α-smooth muscle actin (SMA) (brown, mesenchymal marker) to evaluate epithelial to mesenchymal transition (EMT), and Mn superoxide dismutase (MnSOD) (pink), NADPH odixase 2 (Nox-2) (brown) and superoxide (red fluorescent) to assess oxidative stress. Experimental conditions are shown along the top while antibody and dyes that were utilized are along the left side of the panels. Aggregate semiquantitative scores are presented in the right panel. Cortical staining intensity was scored on a scale of 0 to 3 (0 no staining, 1 mild, 2 moderate, 3 intense) for E-cadherin (distal tubules), α-SMA (interstitium), MnSOD (tubules), Nox-2 (interstitium) and superoxide (tubulointerstitium). Five high magnification fields were evaluated per kidney (five kidneys in each group) and results were expressed as mean and standard error bars.