Juglone reduced fibrogenesis after unilateral ureteral obstruction (UUO). Male Lewis rats (3 months old) underwent UUO of the left kidney for 2 weeks. There were three groups receiving vehicle or juglone (0.25 mg/kg/day or 1 mg/kg/day) for 2 weeks starting the day of surgery. (a) Immunoblot analyses of left kidney from control or juglone-treated rats for the proteins shown along the left. (b) Multiple (n = 3) immunoblots were quantitated and signals expressed as arbitrary units after normalization to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) signal.