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Figure 1 | Fibrogenesis & Tissue Repair

Figure 1

From: Evaluation of intracellular signalling pathways in response to insulin-like growth factor I in apoptotic-resistant activated human hepatic stellate cells

Figure 1

Hepatic stellate cell extracts (20 μg proteins) were run on 10% sodium dodecyl sulphate polyacrylamide gel electrophoresis gel and analysed by Western blot for pSer 473-Akt. Fifteen minutes of incubation with 100 ng/ml of insulin-like growth factor I (IGF-I) induced phosphorylation of c-Akt in Ser 473 (lane 5 panel A and lane 4 panel B). This effect was PI-3K dependent since it was blocked by pre-incubation (30 minutes) of hepatic stellate cells with 100 nM WMN (panel A) or 100 μM LY294002 (panel B), two established inhibitors of PI-3K. Platelet-derived growth factor was used as a positive control for p-Akt and DES-(1–3) IGF-I was used as a positive control for IGF-I. Barograms summarise the results obtained in three independent experiments: * P < 0.05 or a higher degree of significance when compared with stimulation with growth factors without inhibitor.

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Fibrogenesis & Tissue Repair

ISSN: 1755-1536

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