Figure 2
Breast cancer invasion in an organotypic gel. A. Schematic representation of an organotypic cell culture. The stroma consists of collagen:matrigel (70:30) and 5 × 105 human fibroblasts (yellow). 1 × 106 breast cancer cells (blue) are plated on the top of the matrix. The organotypic is then cultures on a grid at the air-liquid interface. B. H & E staining of a section through an organotypic culture 9 days after seeding breast cancer cells (MDA-MB-231) on the top. The image shows breast cancer cells remaining on top of the organotypic culture as well as cells invaded into the stroma.