Figure 1

Organotypic culture of skin equivalent. A. Schematic representation of a skin equivalent organotypic. The stroma consists of collagen and human fibroblasts (5 × 10⁵), with keratinocytes (1 × 10⁶) plated on the top. B. Example of immuno-staining for the differentiation markers Keratin14 and Involucrin in a 10 day old culture. C. A timecourse of wound healing in an organotypic culture using HaCaT human keratinocytes. A 5 mm wound was created using a punch biopsy and the epidermis (pink) of the organotypic was removed using forceps. Wound closure is shown at different time points (0, 2, 4, 7 days post wound).