Transplantation of genetically engineered cardiac fibroblasts producing recombinant human erythropoietin to repair the infarcted myocardium

Table 1 EPO biological activity in vitro

Culture	EPO ELISA (mIU/ml)	EPO activity assay (TF-1) (mIU/ml)	Activity ratio (activity/protein)
Mock BALB/3T3	0	0	-
Transduced BALB/3T3	10.74 ± 0.78	373.3 ± 37.12*	×35
Mock neonatal fibroblasts	0	0	-
Transduced neonatal fibroblasts	51.24 ± 11.17	576.7 ± 186.7*	×12

RhEPO protein levels: the presence of rhEPO in transduced culture supernants was analyzed by a commercial ELISA kit. RhEPO biologic activity (TF-1 cell proliferation assay): EPO biological activity was analyzed using the EPO-sensitive human erythroleukemia cell line TF-1, in which proliferation and metabolic activity is EPO-dependent. *Significantly more active than rhEPO standard (Eprex), p < 0.05.

ISSN: 1755-1536