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Table 1 EPO biological activity in vitro

From: Transplantation of genetically engineered cardiac fibroblasts producing recombinant human erythropoietin to repair the infarcted myocardium

Culture EPO ELISA (mIU/ml) EPO activity assay (TF-1) (mIU/ml) Activity ratio (activity/protein)
Mock BALB/3T3 0 0 -
Transduced BALB/3T3 10.74 ± 0.78 373.3 ± 37.12* ×35
Mock neonatal fibroblasts 0 0 -
Transduced neonatal fibroblasts 51.24 ± 11.17 576.7 ± 186.7* ×12
  1. RhEPO protein levels: the presence of rhEPO in transduced culture supernants was analyzed by a commercial ELISA kit. RhEPO biologic activity (TF-1 cell proliferation assay): EPO biological activity was analyzed using the EPO-sensitive human erythroleukemia cell line TF-1, in which proliferation and metabolic activity is EPO-dependent. *Significantly more active than rhEPO standard (Eprex), p < 0.05.