|
Culture
|
EPO ELISA (mIU/ml)
|
EPO activity assay (TF-1) (mIU/ml)
|
Activity ratio (activity/protein)
|
|---|
|
Mock BALB/3T3
|
0
|
0
|
-
|
|
Transduced BALB/3T3
|
10.74 ± 0.78
|
373.3 ± 37.12*
|
×35
|
|
Mock neonatal fibroblasts
|
0
|
0
|
-
|
|
Transduced neonatal fibroblasts
|
51.24 ± 11.17
|
576.7 ± 186.7*
|
×12
|
- RhEPO protein levels: the presence of rhEPO in transduced culture supernants was analyzed by a commercial ELISA kit. RhEPO biologic activity (TF-1 cell proliferation assay): EPO biological activity was analyzed using the EPO-sensitive human erythroleukemia cell line TF-1, in which proliferation and metabolic activity is EPO-dependent. *Significantly more active than rhEPO standard (Eprex), p < 0.05.
